Hematopoietic stem cells (HSCs), still represent a certain mystery in biology, have a unique property of dividing into equal cells\nand repopulating the hematopoietic tissue. This potential enables their use in transplantation treatments. The quality of the HSC\ngrafts for transplantation is evaluated by flow cytometric determination of the CD34+ cells, which enables optimal timing of the first\napheresis and the acquisition of maximal yield of the peripheral blood stem cells (PBSCs). To identify a more efficient method for\nevaluating CD34+ cells, we compared the following alternative methods with the reference method: hematopoietic progenitor cells\n(HPC) enumeration (using the Sysmex XE-2100 analyser), detection of CD133+ cells, and quantification of aldehyde dehydrogenase\nactivity in the PBSCs. 266 aphereses (84 patients) were evaluated. In the preapheretic blood, the new methods produced data that\nwere in agreement with the reference method. The ROC curves have shown that for the first-day apheresis target, the optimal\npredictive cut-off value was 0.032 cells/mL for the HPC method (sensitivity 73.4%, specificity 69.3%). HPC method exhibited a\ndefinite practical superiority as compared to other methods tested. HPC enumeration could serve as a supplementary method for\nthe optimal timing of the first apheresis; it is simple, rapid, and cheap.
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